rabbit polyclonal igf ii  (Santa Cruz Biotechnology)

Journal: Internal Medicine

Article Title: A Hypoglycemia-inducing Giant Borderline Phyllodes Tumor Secreting High-molecular-weight Insulin-Like Growth Factor II: Immunohistochemistry and a Western Blot Analysis

doi: 10.2169/internalmedicine.9287-17

Figure Lengend Snippet: The microscopic findings [Hematoxylin and Eosin (H&E) staining and immunohistochemical staining of the resected tumor]. H&E staining (A) Low-power (×40) and (B) high-power (×400) views showed moderate stromal hypercellularity with mild nuclear atypia and mild pleomorphism of the spindle cells. Focal mildly atypical epithelial hyperplasia was also noted. Stromal overgrowth was absent. Immunohistochemical staining of (C) the phyllodes tumor and (D) normal breast tissue using rabbit polyclonal anti-insulin-like growth factor II (IGF-II) antibodies. The tumor cells, but not the normal breast tissue, were diffusely immunopositive for IGF-II.

Article Snippet: They were probed with rabbit polyclonal anti-IGF-II antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Staining, Immunohistochemical staining

Journal: Internal Medicine

Article Title: A Hypoglycemia-inducing Giant Borderline Phyllodes Tumor Secreting High-molecular-weight Insulin-Like Growth Factor II: Immunohistochemistry and a Western Blot Analysis

doi: 10.2169/internalmedicine.9287-17

Figure Lengend Snippet: Western blotting of high-molecular-weight IGF-II. Lane 1, serum from a healthy control; lane 2, serum from the patient collected preoperatively; lane 3, serum obtained 3 days after the resection of the tumor; lane 4, phyllodes tumor tissue. Large amounts of high-molecular-weight IGF-II were detected in the serum collected preoperatively (lane 2) and in the tumor tissue (lane 4) but not in serum collected postoperatively (lane 3) or a serum sample from a healthy control (lane 1).

Article Snippet: They were probed with rabbit polyclonal anti-IGF-II antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Western Blot, High Molecular Weight, Control

Journal: Oncotarget

Article Title: IGF-II induced by hepatitis B virus X protein regulates EMT via SUMO mediated loss of E-cadherin in mice

doi: 10.18632/oncotarget.10922

Figure Lengend Snippet: A. and C. Relative mRNA expression of IGF-II in WT and HBx mouse livers (A) Mean ± SD ; * P< 0.05, ** P< 0.01, n =3, HepG2-Mock and HepG2-HBx cells (C) Mean + SD ; * P< 0.05, n =5-8. B. Immunohistochemistry images of 3-week-old WT and HBx-mouse livers stained with IGF-II. Original magnifications 40X. Data are representative of 3-5 mice per group. D. Secreted IGF-II protein levels from HepG2-Mock and HBx cells were measured. Mean ± SD ; * P< 0.05, n =6.

Article Snippet: Samples were incubated overnight at 4 °C with anti-IGF-II rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology, CA, USA).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Oncotarget

Article Title: IGF-II induced by hepatitis B virus X protein regulates EMT via SUMO mediated loss of E-cadherin in mice

doi: 10.18632/oncotarget.10922

Figure Lengend Snippet: Mean ± SD ; * P< 0.05, n =6. B. Cell growth was measured in HepG2-Mock cells treated with conditioned medium from HepG2-Mock (CM-Mock), and HBx (CM-HBx). Mean ± SD ; * P < 0.05, n=6. C. Cell numbers were quantified in HepG2-Mock and HBx cells with or without anti-IGF-II. Mean ± SD ; ** P< 0.01, n =4. D. Multilayer growth of HepG2-HBx cells were reversed to monolayer growth with anti-IGF-II.

Article Snippet: Samples were incubated overnight at 4 °C with anti-IGF-II rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology, CA, USA).

Techniques: